Diaphonization: Enhancing Efforts Toward Achieving SDGs 10, 14, and 15
Suvranil Dutta
Department of Botany, Kalyani University, Kalyani, India
https://orcid.org/0009-0004-1853-0605
Pronoy Mukherjee
Department of Zoology, Rishi Bankim Chandra College, Naihati, India https://orcid.org/0000-0002-4901-0141
Anwesha Mitra
Department of Botany, Kalyani University, Kalyani, India https://orcid.org/0009-0009-1457-9838
Bibhas Guha
Department of Zoology, Netaji Subhas Open University, Sector-1, Salt Lake City, Kolkata-64
Biswajit (Bob) Ganguly
Noble International University (NIU), USA
Tanmay Sanyal
Department of Zoology, Krishnagar Govt. College, Krishnagar, India https://orcid.org/0000-0002-0046-1080
Published online:8 August, 2024
DOI: https://doi.org/10.52756/boesd.2024.e03.013
Keywords: Diaphonization, conservation, clearing & staining, Morphology, Phylogeny, Sustainable Development
Abstract:
Diaphonization, also known as clearing and staining, is an invaluable technique for visualizing and studying the skeletal structures of animals. This study explores the application of diaphonization to examine bone development and soft tissues and determine causes of death in various animal species. By rendering tissues transparent and selectively staining bones and cartilage, diaphonization allows for detailed observation of skeletal morphology without dissection, thus preserving the integrity of specimens. This method enhances the understanding of morphological and anatomical structures and aids in determining the phylogenetic relationships between species. Diaphonization directly contributes to achieving Sustainable Development Goal (SDG) 4 by improving educational tools, indirectly supports SDG 10 by reducing educational inequalities, and SDG 14 and 15 by providing knowledge about the habitat of different species. Additionally, it plays a significant role in research, conservation, forensic science, and embryological studies.
References:
- Chitra, V., & Sharon, S. E. (2020). Diaphonization of the Ovariectomized Laboratory Animal. Research Journal of Pharmacy and Technology, 13(5), 2228-2232.
- Cumley, R. W., Crow, J. F., & Griffen, A. B. (1939). Clearing specimens for the demonstration of bone. Stain Technology, 14(1), 7-11.
- Dawson, A. B. (1926). A note on the staining of the skeleton of cleared specimens with alizarin red S. Stain Technology, 1(4), 123-124.
- Lee, M. L. H. (1963). The intraosseous arterial pattern of the carpal lunate bone and its relation to avascular necrosis. Acta Orthopaedica Scandinavica, 33(1-4), 43-55.
- Lipman, H. J. (1935). Staining the skeleton of cleared embryos with alizarin red S. Stain Technology, 10(2), 61-63.
- Mukherjee, P., Saha, A., Sen, K., Erfani, H., Madhu, N. R., & Sanyal, T. (2022). Conservation and prospects of Indian Lacustrine fisheries to reach the Sustainable Developmental Goals (SDG 17). A Basic Overview of Environment and Sustainable Development, pp. 98-116.
- Rehman, K., Khan, F. R., & Habib, S. (2015). Diaphonization: a recipe to study teeth. J Contemp Dent Pract, 16(3), 248-51.
- Rueda-Esteban, R. J., Varona, J. P., López-McCormick, J. S., & Restrepo, J. D. H. (2017). Diaphanization: A Standardized Protocol for Non-Fetal Tissue Preservation. International Journal of Morphology, 35(2).
- Susaki, E. A., Shimizu, C., Kuno, A., Tainaka, K., Li, X., Nishi, K., … & Ueda, H. R. (2020). Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues. Nature Communications, 11(1), 1982.
Get PDF
[Volume: 3]
How to Cite
Suvranil Dutta, Pronoy Mukherjee, Anwesha Mitra, Bibhas Guha, Biswajit (Bob) Ganguly and Tanmay Sanyal (2024). Diaphonization: Enhancing Efforts Toward Achieving SDGs 10, 14, and 15 © International Academic Publishing House (IAPH), Dr. Nithar Ranjan Madhu, Dr. Tanmay Sanyal, Dr. Koushik Sen, Professor Biswajit (Bob) Ganguly and Professor Roger I.C. Hansell (eds.), A Basic Overview of Environment and Sustainable Development [Volume: 3], pp. 210-216. ISBN: 978-81-969828-3-6
DOI: https://doi.org/10.52756/boesd.2024.e03.013
SHARE WITH EVERYONE